Effect of Low Rabll Expression on the Proliferation and Invasion of Human Bladder Cancer Cells

VernacularTitle:抑制Rab11表达对人膀胱癌细胞增殖和侵袭的影响
Author: Xue GONG ¹ ; Liu YU ; Xingwang ZHU ; Jia LIU ; Yili LIU ; Ping WANG
Author Information

1. 中国医科大学附属第四医院泌尿外科
Keywords: Rabll; bladder urothelial carcinoma; proliferation; invasion
From: Journal of China Medical University 2018;47(3):247-250,259
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of inhibition of Rabll expression on the proliferation and invasion of human bladder cancer cell line T24. Methods T24 cells were transfected with Rabll siRNA, and RNA interference efficiency was determined by performing Western blotting. The effect of inhibition of Rabll expression on cell proliferation, cell cycle progression, and cell invasion was analyzed by performing CCK8, cell cycle detection, and Matrigel invasion assays, respectively. Expression of cell cycle-related proteins cyclin D1 and cyclin E and invasion-related protein matrix metalloproteinase 9 (MMP9) was determined by performing Western blotting and RT-PCR. Results Inhibition of Rabl 1 expression inhibited the proliferation and invasion of bladder cancer cells and downregulated the expression of cell cycle-related proteins cyclin D1 and cyclin E and invasion-related protein MMP9. Conclusion Our results suggest that Rabl 1 functions as a tumor protein and is involved in the progression of bladder cancer.