Elevation of Divalent Metal Transporter 1 Protein in the Cerebellar Cortex of the APP/PS1 Transgenic Mouse

VernacularTitle:DMT1在APP/PS1转基因小鼠小脑皮质中表达上调
Author: Siqi WANG ¹ ; Xinlu LI ; Geng LIN ; Zhuo WANG ; Xiaofeng CHENG ; Tongtong LIU ; Wei ZHENG
Author Information

1. 中国医科大学临床一系
Keywords: divalent metal transporter 1; amyloid beta; APP/PS1 transgenic mouse; cerebellum; Alzheimer ' s disease
From: Journal of China Medical University 2018;47(3):193-197
CountryChina
Language:Chinese
Abstract: Objective To investigate the distribution of divalent metal transporter 1 (DMT1) in the cerebellum of APP/PS1 transgenic mouse. Methods Immunohistochemistry, immunofluorescence, and confocal laser scanning microscopy were used to analyze the relationship between DMTl and amyloid beta (Aβ) and their distribution in senile plaques. Western blotting was used to analyze DMT1 protein level in the APP/PS1 transgenic mouse cerebellum. Results DMTl and Aβ were mainly located in the amyloid plaques, which were predominately located in the molecular layer of the cerebellar cortex of the transgenic mouse. Only a few plaques could be seen in the Purkinje cell layer and granular layer. Confocal laser microscopy revealed the DMTl and Aβ were co-localized in senile plaques. Conclusion The abundant expression of DMTl protein suggests that DMTl and the divalent metal ions that it transports might be involved in the formation of Aβ senile plaques and other pathological processes in the cerebellum in Alzheimer' s disease.