Study of the Epithelial Mesenchymal Transition between Liver Cirrhosis and Liver Cancer in Mice
10.12007/j.issn.02584646.2018.02.014
- VernacularTitle:小鼠肝硬化—肝癌上皮间质转化的研究
- Author:
Hongli SUN
1
Author Information
1. 中国医科大学附属盛京医院胆道血管外科
- Keywords:
liver cirrhosis model;
liver cancer model;
E-cadherin;
β3-integrin;
vimentin;
fibronectin;
epithelial-mesenchymal transition
- From:Journal of China Medical University
2018;47(2):157-161
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish animal models facilitating observation of the process of epithelial-mesenchymal transition between liver cirrhosis and liver cancer. Methods Liver cirrhosis and liver cancer models were established in mouse and subsequently assessed using HE and immunohistochemical staining. Results The success rate of the liver cirrhosis model was 100％ and the fatality rate was 30％. The success rate of the liver cancer model was also 100％ and the fatality rate was 40％. In the liver cirrhosis and liver cancer models,the positive expression rates were,respectively,46. 7％ and 7. 1％ for E-cadherin,40. 0％ and 7. 1％ for β3-integrin,13. 6％ and 40. 0％ for vimentin,and 20. 0％ and 57. 1％ for fibronectin. Conclusion In mouse models of liver cirrhosis and liver cancer,low expression levels of E-cadherin and β3-integrin in addition to high expression levels of fibronectin and vimentin are related to the epithelial-mesenchymal transition between liver cancer and cirrhosis,and may be important indicators for clinical detection.
