Expression and Action Mechanism of miR-98 in Colon Cancer Tissues
10.12007/j.issn.02584646.2018.01.003
- VernacularTitle:结肠癌组织中miR-98的表达及作用机制
- Author:
Junjiang WANG
1
;
Tianyu ZHOU
;
Yang ZHANG
;
Mingxin LIU
Author Information
1. 辽宁中医药大学附属医院肛肠科
- Keywords:
miR-98;
colon cancer;
proliferation;
apoptosis;
survival duration
- From:Journal of China Medical University
2018;47(1):9-12
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and action mechanism of miR-98 in colon cancer tissues. Methods Tumor tissues and adjacent tissues were collected from 40 patients with colorectal cancer. The expression of miR-98 in tumor tissues and adjacent tissues was detected by real-time PCR. miR-98 was overexpressed or silenced in cells,and the effects on proliferation,cell cycle,and apoptosis were analyzed using MTT,flow cytometry,and Hoechst 33258 assays. Results Real-time PCR showed that the expression of miR-98 in tumor tissues was lower than that in adjacent tissues (P = 0.022). The survival rate of patients with lower miR-98 expression was shorter than that of patients with higher miR-98 expression. The MTT assay showed that miR-98 overexpression inhibited the proliferation of HCT116 cells. Hoechst 33258 staining showed that the overexpression of miR-98 could inhibit the cell cycle and promote the apoptosis of HCT116 cells. Conclusion miR-98 can inhibit the proliferation and promote the apoptosis of colon cancer cells. The expression of miR-98 is closely related to the survival of patients with colon cancer.
