Distribution of the Glycosaminoglycans in the Bruch`s Membrane and Sensory Retina : Histochemical Electromic roscopic Findings with Ruthenium Red.
- Author:
Won Ki LEE
1
;
Myung Kyoo KO
;
Dong Seob KIM
Author Information
1. Department of Ophthalmology, Chung-Ang Gil Medical Center, College of Medicine, Gachon University, Inchon, Korea.
- Publication Type:Original Article
- Keywords:
Glycosaminoglycan;
Ruthenium red;
Bruch`s membrane;
retina;
Ultrastructure
- MeSH:
Basement Membrane;
Collagen;
Cytoplasm;
Extracellular Space;
Filtration;
Glycosaminoglycans*;
Humans;
Membranes*;
Photoreceptor Cells;
Retina*;
Retinal Neurons;
Retinal Pigment Epithelium;
Ruthenium Red*;
Ruthenium*
- From:Journal of the Korean Ophthalmological Society
1999;40(7):1877-1883
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The distribution of glycosaminoglycans in Bruch`s membrane and sensory retina of human eye was evaluated with histochemical electron microscope using 0.05%ruthenium red (RR). Seven donated or enucleated eyeballs without chorioretinal disease were used.Electron-dense RR reactive materials were identified as fine discrete granules along the basement membrane of the retinal pigment epithelium, in the inner and outer collagen layer and central elastic layer of Bruch 's membrane, and in the interphotorecep- tor matrix. In addition, they were observed at the cytoplasmic wall of photoreceptor cells, their intercellular spaces, and the internal limiting membrane. These results demonstrated that GAGs are widely distributed within the interphotoreceptor matrix, Bruch`s membrane, and intercellular spaces of retinal neurons. They are considered to function as a mechanical supporter as well as a selective filtration barrier through which the metabolitespass.
