Effects of miR-218-1-3p on Cell Proliferation, Cycle, and Apoptosis of Non-small Cell Lung Cancer
10.12007/j.issn.0258-4646.2017.11.005
- VernacularTitle:miR-218-1-3p对非小细胞肺癌细胞增殖、周期及凋亡的影响
- Author:
Lan LUAN
1
;
Tiange ZHANG
;
Bin HAN
;
Wenting LUO
;
Fei WU
;
Xin LIAO
;
Junjie ZHANG
;
Yang BAI
;
Xinyu CHENG
;
Meng TENG
;
Cuifang WANG
Author Information
1. 沈阳医学院附属中心医院病理科
- Keywords:
miR-218-1-3p;
non-small cell lung cancer;
proliferation;
cycle;
apoptosisin
- From:
Journal of China Medical University
2017;46(11):980-983,994
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of miR-218-1-3p on the proliferation,cycle,and apoptosis of A549 cells in non-small-cell lung cancer.Methods miR-218-1-3p was transfected into non-small cell lung cancer A549 cells by LipofectamineTM 2000 Reagent,and the expression of miR-218-3p was detected by real-time PC R.Invasion and migration were assayed using the Transwell method.The effect of miR-218-1-3p on the proliferation of A549 cells was assayed by the MTS method.Changes in the cell cycle and apoptosis of A549 cells transfected with miR-218-1-3p was detected by flow cytometry.Changes in indicators related to cell proliferation,cycle,and apoptosis were detected by fluorescence quantitative PCR.Results Compared to the control group,the cell proliferation of A549 cells was significantly inhibited (P ＜ 0.05) and the proportion of cells in the S and G2-M phases was significantly decreased when miR-218-1-3p was up-regulated.In addition,compared with the control group,the early apoptotic rate was significantly increased by up-regulating miR-218-1-3p.We further detected indicators related to cell proliferation,cycle,and apoptosis and found that CYCLIN-D1 and BCL-2 were significantly downregulated.Conclusion miR-218-1-3p may inhibit proliferation,induce cell cycle arrest,and promote cell apoptosis of non-small cell lung cancer A549 cells by regulating CYCLIN-D 1 and BCL-2.
