Simultaneous Determination of Loganic Acid and Isoscoparin in Sanwei Longdanhua Tablets by HPLC
10.6039/j.issn.1001-0408.2018.20.16
- VernacularTitle:HPLC法同时测定三味龙胆花片中马钱苷酸和异金雀花素的含量
- Author:
Yueyan AI
1
;
Xueyong ZHAO
;
Lin FU
;
Xiaolian BIAN
;
Rui GU
;
Jingbo ZHANG
;
Nancuo
;
Xiraonamu
Author Information
1. 成都中医药大学民族医药学院
- Keywords:
HPLC;
Sanwei longdanhua tablets;
Loganic acid;
Isoscoparin;
Content determination
- From:
China Pharmacy
2018;29(20):2810-2813
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of loganic acid and isoscoparin in Sanwei longdanhua tablets. METHODS:HPLC method was adopted. The determination was performed on Inertsil ODS-3 column with mobile phase consisted of methanol-0.2% phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength was set at 240 nm,and column temperature was 30 ℃. The sample size was 10 μL. RESULTS:The linear range were 0.040 08-4.008 0 μg(r＝0.999 9)for loganic acid and 0.021 96-2.196 0 μg(r＝0.999 9)for isoscoparin. The quantitative limits were 0.160 32 and 0.087 8 ng/mL,and detection limits were 0.080 16 and 0.043 92 ng/mL. RSDs of precision,stability and reproducibility tests were all lower than 2%. The recoveries were 103.07%-104.26%(RSD＝0.52%,n＝6) and 95.57%-99.61%(RSD＝1.55%,n＝6). CONCLUSIONS:The method is simple,accurate and suitable for simultaneous determination of loganic acid and isoscoparin in Sanwei longdanhua tablets.
