Study on Inhibitory Effects of Recombinant Adenovirus Ad-GFP-C197 on 3 Kinds of Tumor Cells
10.6039/j.issn.1001-0408.2018.20.14
- VernacularTitle:重组腺病毒Ad-GFP-C197对3种肿瘤细胞的抑制作用研究
- Author:
Xian WU
1
;
Ying HUANG
;
Shujie LI
;
Pingping DUAN
;
Pengcheng MI
;
Wenying CHEN
Author Information
1. 南方医科大学药学院
- Keywords:
hTERT;
Telomerase inhibitor;
Recombinant adenovirus;
Ad-GFP-C197;
Tumor cell;
Cell proliferation
- From:
China Pharmacy
2018;29(20):2800-2804
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the inhibitory effects of recombinant adenovirus Ad-GFP-C197, which prepared by adenovirus vector system-loading human telomerase reverse transcriptase(hTERT)C fragment(C197),on the proliferation of 3 kinds of tumor cells in vitro. METHODS:Ad-GFP-C197 was amplified and purified with HEK293 cells. Human gastric cancer cells SGC7901,human breast cancer cells MCF7 and human colorectal cancer cells CaCO2 were infected by Ad-GFP-C197 respectively. Using blank adenovirus carrier (Ad-GFP) as reference,the protein expression of C197 in 3 kinds of tumor cells infected by Ad-GFP-C197 was detected by Western blot assay. The inhibitory effects of Ad-GFP-C197 on 3 kinds of tumor cells were detected by MTT assay. The cell proliferation curve was drawn and the proliferation inhibition rate was calculated. RESULTS:The protein expression of C197 was not detected in 3 kinds of tumor cells infected by Ad-GFP,while significant protein expression of C197 was found in above cells infected by Ad-GFP-C197. The proliferation curves of the 3 kinds of tumor cells infected by Ad-GFP-C197 were significantly inhibited with the time extended,and the proliferation inhibitory rate reached 37.31%-41.42%. CONCLUSIONS:Ad-GFP-C197 shows significant inhibitory effects on the proliferation of SGC7901,MCF7 and CaCO2 cells, which is rapid to make up for the slow effect of other telomerase inhibitors.
