Study of Transfer Rate of Standard Decoction of Dipsacus asper Decoction Pieces
10.6039/j.issn.1001-0408.2018.20.10
- VernacularTitle:续断饮片标准汤中川续断皂苷Ⅵ转移率的研究
- Author:
Junshan LI
1
;
Yunfeng CHANG
;
Hao MA
;
Liying NIU
;
Zhenjiang LI
Author Information
1. 神威药业集团有限公司
- Keywords:
Dipsacus asper;
Decoction pieces;
Standard decoction;
HPLC;
Asperosaponin Ⅵ;
Transfer rate
- From:
China Pharmacy
2018;29(20):2782-2785
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study transfer rate of asperosaponinⅥ in standard decoction of Dipsacus asper decoction pieces. METHODS:The content of asperosaponin Ⅵ in Dipsacus asper decoction pieces and its standard decoction was determined by HPLC. The determination was performed on SinoChrom ODS-AP with mobile phase consisted of acetonitrile-water(30∶70,V/V)at the flow rate of 1.0 mL/min. The detection wavelength was set at 212 nm,and column temperature was 30℃. The sample size was 10 μL. By the ingredients content obtained,the transfer rate of asperosaponin Ⅵ was calculated during decoction piece to standard decoction. RESULTS:The linear range of asperosaponin Ⅵ was 0.484-4.84 μ g(r＝0.999 9). RSDs of precision,stability and reproducibility tests were all lower than 2%. The limits of quantification and detection were 0.3 and 0.1 μ g,respectively. The average recoveries in D. asper decoction pieces and standard decoction were 95.13% -100.22%(RSD＝1.78%,n＝6), 97.07%-100.08%(RSD＝0.98%,n＝6). RSD of durability test was lower than 1%.The transfer rate of asperosaponin Ⅵ in standard decoction of D. asper decoction pieces ranged 26.3%-49.5%. CONCLUSIONS:The method is simple,accurate,precise, stable,reproducible and durable,and can be used for transfer rate of asperosaponin Ⅵ in standard decoction of D. asper decoction pieces.
