Study on the Effect and Mechanism of Euphornin Inducing the Apoptosis of Cervical Cancer Hela Cells
10.6039/j.issn.1001-0408.2018.20.08
- VernacularTitle:大戟苷诱导人宫颈癌Hela细胞凋亡及其作用机制研究
- Author:
Deli ZHANG
1
;
Xiaoqiang LI
;
Yinliang BAI
;
Rongxia HE
;
Yinfeng LYU
;
Huifang WEN
;
Li WEI
Author Information
1. 兰州大学第二医院药学部
- Keywords:
Euphornin;
Cervical cancer;
Hela cell;
Apoptosis;
Mechanism
- From:
China Pharmacy
2018;29(20):2773-2776
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study induction effect of euphornin on the apoptosis of cervical cancer Hela cells and its mechanism. METHODS:The cervical cancer Hela cells were divided into blank control group,cisplatin group(positive control, 10 mg/L) and euphornin low-dose,medium-dose and high-dose groups (50,100,200 mg/L). They were treated with relevant medicine. The inhibitory effect of Hela cells proliferation was tested by MTT assay after 24,48,72 h of medicine treatment. The apoptotic rate of Hela cells was measured by flow cytometry after 48 h of medicine treatment. Morphology of nucleus was detected by Hoechst 33258 staining. The protein expression of Cyt-C,Bcl-2,Bax,Caspase-3,Caspase-8,Caspase-9 and Caspase-10 were detected by Western blot assay. RESULTS:Compared with blank control group,inhibitory rate of cell proliferation and cell apoptosis rate were increased significantly in cisplatin group and euphornin groups(P＜0.05 or P＜0.01),and obvious staining, deformation,shrinking,fragmentation or apoptotic bodies was found in nucleus. Compared with blank control group,the protein expression levels of Cyt-C,Caspase-8 and Caspase-9 in euphornin low-dose,medium-dose and high-dose groups were increased significantly,while the protein expression level of Bcl-2 and Bcl-2/Bax ratio were decreased significantly(P＜0.05 or P＜0.01);the protein expression levels of Bax,Caspase-3 and Caspase-10 in euphornin medium-dose and high-dose groups were increased significantly(P＜0.05 or P＜0.01). CONCLUSIONS:Euphornin can significantly inhibit the proliferation of Hela cell and promote cell apoptosis,the effect of which will be achieved by activating the Caspase-dependent mitochondrion apoptosis pathway.
