Study on HPLC Fingerprint of Miao Medicine Fufukang Spray
10.6039/j.issn.1001-0408.2018.11.21
- VernacularTitle:苗药妇肤康喷雾剂的HPLC指纹图谱研究
- Author:
Xiu LIAO
1
;
Jingjing XU
;
Yan YANG
;
Lilang LI
;
Ke WANG
;
Qiji LI
;
Xiaosheng YANG
Author Information
1. 贵州省中国科学院天然产物化学重点实验室
- Keywords:
Fufukang spray;
HPLC;
Fingerprint;
6′-hydroxyl-justicidin B;
Justicidin B
- From:
China Pharmacy
2018;29(11):1529-1532
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish HPLC fingerprint of Miao medicine Fufukang spray. METHODS:The determination was performed on Thermo Hypersil GOLD C18 column with mobile phase consisted of acetonitrile-0.2％ glacial acetic acid (gradient elution) at the flow rate of 0.6 mL/min. The detection wavelength was set at 262 nm,and column temperature was 25 ℃. The sample size was 10 μL. Using 6′-hydroxyl-justicidin B as reference control,HPLC chromatograms of 10 batches of samples were drawn. Similarity evaluation system software(2004 A edition)of TCM fingerprint was used to evaluate the similarity and determine common peaks of 10 batches of samples. The established method was used to determine the contents of 6′-hydroxyl-justicidin B and justicidin B in 10 batches of samples. RESULTS:There were 12 common peaks in HPLC fingerprints of 10 batches of samples with similarity>0.90. HPLC fingerprint showed a good consistency with the control fingerprint. The contents of 6′-hydroxyl-justicidin B and justicidin B in 10 batches of samples were 1.64-5.84,0.83-2.78 mg/100 mL,respectively. CONCLUSIONS:The established method is simple,accurate,reliable and suitable for quality evaluation of Fufukang spray.
