Simultaneous Determination of Berberine Hydrochloride and Baicalin in Jianpi Zhixiening Granules by HPLC-switching Walvelength Method
10.6039/j.issn.1001-0408.2018.10.07
- VernacularTitle:HPLC切换波长法同时测定健脾止泻宁颗粒中盐酸小檗碱和黄芩苷的含量
- Author:
Chuanjun HUANG
1
;
Li YANG
;
Yong MEI
;
Lei LUO
;
Shanshan LYU
;
Bocheng ZENG
;
Tao LONG
;
Feng WANG
;
Juan ZUO
;
Kaichao YUAN
;
Pan TANG
;
Feng ZHU
;
Bo CHEN
;
Zhiwen QIAO
Author Information
1. 湖北省中医院药事部
- Keywords:
Jianpi zhixiening granules;
HPLC switching walvelength method;
Berberine hydrochloride;
Baicalin;
Content determination
- From:
China Pharmacy
2018;29(10):1324-1327
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the method for simultaneous determination of berberine hydrochloride and baicalin in Jianpi zhixiening granules. METHODS:HPLC switching walvelength method was adopted. The determination was performed on Hypersil BDS C18 column with mobile phase consisted of methanol-0.45％ phosphoric acid solution-triethylamine(50:49:1,V/V/V) at the flow rate of 1.0 mL/min. The detection wavelength was set at 265 nm(berberine hydrochloride)and 280 nm(baicalin). The column temperature was set at 30 ℃,and sample size was 10 μL. RESULTS:The linear range of berberine hydrochloride and baicalin were 60.3-312.8 ng(r=0.9997)and 81.5-368.9 ng(r=0.9999). The limits of quantitation were 0.6668,0.7740 ng,andthe limits of detection were 0.2226,0.2580 ng,respectively. RSDs of intermediate precision,stability and repeatability tests were all lower than 1.0％. The recoveries were 96.48％-99.30％(RSD=1.06％,n=6) and 95.20％-99.39％(RSD=1.66％,n=6), respectively. RSDs of durability test were all lower than 2.0％. CONCLUSIONS:The method is simple, precise, stable, reproducible,accurate and durable. It can be used for simultaneous determination of berberine hydrochloride and baicalin in Jianpi zhixiening granules.
