Identification and Analysis of Tuberostemonine Metabolites in Rats by UPLC-Q-TOF/MS

VernacularTitle:UPLC-Q-TOF/MS法分析鉴定对叶百部碱在大鼠体内的代谢产物
Author: Wei DONG ¹ ; Qiuping WU ; Xin LIANG ; Tao CUI ; Hong PAN
Author Information

1. 齐齐哈尔医学院药学院
Keywords: Tuberostemonine; UPLC-Q-TOF/MS; Metabolite; Identification
From: China Pharmacy 2018;29(9):1218-1221
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To analyze and identify the metabolites of tuberostemonine in rats plasma,urine and feces,and to clarify metabolic pathway of tuberostemonine. METHODS:Rats were randomly divided into blank group(0.3% carboxymethyl cellulose)and medication group(tuberostemonine 50 mg/kg,i.g.),with 6 rats in each group.The plasma of rats was collected 0.25, 0.5,1,2,4,6,12 h after intragastric administration to prepare samples. Urine and feces were collected 0-12 h and 12-24 h after administration to prepare samples. UPLC-Q-TOF/MS and software of Triple TOFTMhigh resolution mass spectrometry were used to analyze and identifiy chemical structure of metabolites in samples. RESULTS:A total of 4 compounds were detected in rat plasma, including one prototype compound and 3 metabolites. 4 metabolites were detected in urine and 2 metabolites in feces. Phase Ⅰmetabolites of tuberostemonine mainly included hydration and hydroxylation. Phase Ⅱ metabolites were not found. CONCLUSIONS:Hydration and hydroxylation are the major metabolic transformation forms of tuberostemonine in rats in vivo.