Determination of Molecular Weight and Content of Bletilla striata Polysaccharide by HPSEC-ELSD
10.6039/j.issn.1001-0408.2018.09.11
- VernacularTitle:HPSEC-ELSD法同时测定白及多糖的分子量和含量
- Author:
Chuan WANG
1
;
Yan ZHANG
;
Nan LI
;
Yali LI
;
Jiangyun LIU
;
Peilie CAI
;
Lili HAO
Author Information
1. 苏州大学医学部药学院
- Keywords:
Bletilla striata;
Polysaccharide;
HPSEC-ELSD;
Molecular weight;
Content
- From:
China Pharmacy
2018;29(9):1198-1201
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To set up a method for determination of molecular weight and content of polysaccharide in Bletilla striata. METHODS:HPSEC-ELSD method was adopted. The determination was performed on TSK-GEL G4000 PWXL column with mobile phase consisted of pure water at the flow rate of 0.6 mL/min.The column temperature was 30 ℃,and sample size was 20 μL. Evaporative light scattering detector was used.The molecular weight and content of polysaccharide in 3 batches of B. striata were established. RESULTS:The linear range of weight average molecular weight of B. striata polysaccharide were 24.17-178.00 kD(R2=0.985 5). The linear range of BT07 content determination were 0.508 0-5.080 mg/mL(R2=0.998 4). The limits of detection and quantitation were 0.116 5 and 0.274 0 mg/mL. RSDs of precision,stability and reproducibility tests were all lower than 2%(n=6 or n=7).Average recoveries rate were 99.16%-100.20%(RSD=0.39%-0.64%,n=9).Average retention time of 3 batches of samples was 14.28 min(RSD=4.35%,n=3),and weight average molecular weight was 23.54 kD(RSD=3.78%, n=3). Polydispersity coefficient D,MW/Mn)ranged 1.463-1.578. Average content of sample was 93.4%(RSD=4.22%,n=3). CONCLUSIONS:HPSEC-ELSD method is simple,rapid,accurate and reliable,which can be used for simultaneous determination of B.striata polysaccharide.
