Study on HPLC Fingerprints of Yigan Yiqi Jieyu Granules
10.6039/j.issn.1001-0408.2018.08.06
- VernacularTitle:乙肝益气解郁颗粒的HPLC指纹图谱研究
- Author:
Yanbing CUI
1
;
Zhixia XING
;
Shuaijun LIN
;
Wei LIU
Author Information
1. 漯河医学高等专科学校药学系
- Keywords:
Yigan yiqi jieyu granules;
HPLC;
Fingerprint;
Paeoniflorin
- From:
China Pharmacy
2018;29(8):1036-1039
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish HPLC fingerprints of Yigan yiqi jieyu granules. METHODS:HPLC method was adopted. The determination was performed on Diamonsil C18column with mobile phase consisted of water-acetonitrile(gradient elution)at flow rate of 1.0 mL/min. The detection wavelength was set at 230 nm,and column temperature was 30 ℃. The sample size was 10 μL. Using paeoniflorin as control,HPLC chromatograms of 10 batches of samples were determined.The similarity was evaluated by using TCM Chromatogram Fingerprint Similarity Evaluation System(2004 A version)to determine common peak. RESULTS:There were 34 common peaks in HPLC fingerprints of 10 batches of samples,with the similarities>0.98. After validation,HPLC chromatograms of 10 batches of were in good agreement with control fingerprint. CONCLUSIONS:Established fingerprint can provide reference for identification and quality evaluation of Yigan yiqi jieyu granules.
