Study on Extraction Technology Optimization of Tibetan Medicine Cortex of Berberis dictyophylla by Ethanol
10.6039/j.issn.1001-0408.2018.07.23
- VernacularTitle:藏药小檗皮的醇提工艺优化研究
- Author:
Lu HAO
1
;
Zhen ZHOU
;
Hui FENG
;
Ya ZHAO
;
Banghua ZHOU
;
Gang FAN
;
Xianrong LAI
Author Information
1. 成都中医药大学民族医药学院
- Keywords:
Tibetan medicine;
Cortex of Berberis dictyophylla;
Ethanol extract;
Uniform design method;
Extraction technology
- From:
China Pharmacy
2018;29(7):958-963
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize extraction technology of cortex of Berberis dictyophylla by ethanol. METHODS:Using the contents of magnoflorine,jatrorrhizine hydrochloride,palmatine hydrochloride and berberine hydrochloride,the amount of extract as evaluation indexes,the effects of ethanol amount,volume fraction of ethanol and extraction time on extraction technology were investigated by uniform design method-comprehensive scoring method. The extraction methods of first time, second time and third time were investigated by 3 times of uniform design test. The optimal schemes of 3 times of extraction test were determined and validation test was conducted,and the transfer rates were calculated. RESULTS:The optimal technology was as follows as coarse powder of cortex of B. dictyophylla,15-fold 75% ethanol,extracting for 2 times,120 min each time. In validation test,the contents of magnoflorine,jatrorrhizine hydrochloride,palmatine hydrochloride and berberine hydrochloride were 58.96,4.82,3.07,23.29 mg/g after B. dictyophylla was extracted by optimization technology for 2 times. The transfer rates were 93.85%,95.02%,96.28%,94.88%,respectively(RSD=3.87%,2.64%,4.00%,3.91%,n=3). CONCLUSIONS:The optimal ethanol reflux extraction technology of cortex of B.dictyophylla is reasonable and feasible with good stability.
