Effects of Polydatin on Protein Expression of MMP-9 and TIMP-1 in Renal Tissue of Renal Fibrosis Model Rats
10.6039/j.issn.1001-0408.2018.07.18
- VernacularTitle:虎杖苷对肾纤维化模型大鼠肾组织中MMP-9和TIMP-1蛋白表达的影响
- Author:
Song WANG
1
;
Xiaoyu ZHAO
;
Yan LIANG
;
Yingjie ZHANG
;
Yuedong QI
Author Information
1. 郑州大学第一附属医院药学部
- Keywords:
Polydatin;
Renal fibrosis;
Unilateral ureteral obstruction;
MMP-9;
TIMP-1
- From:
China Pharmacy
2018;29(7):936-940
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of polydatin on protein expression of MMP-9 and TIMP-1 in renal tissue of renal fibrosis model rats,and to investigate the mechanism of delaying renal fibrosis in rats. METHODS:A total of 40 rats were randomly divided into sham operation group(normal saline),model group(normal saline),polydatin group(100 mg/kg)and benazepril group(positive control,5 mg/kg). Except for sham operation group,renal fibrosis rat model was induced by unilateral ureteral obstruction in other groups. 1 h after surgery,each group was given relevant medicine intragastrically,once a day.After 4 weeks of consecutive treatment,the contents of β 2-microglobulin(β 2-MG)and N-acetyl-β-D-glucosaminidase(NAG)in urine, serum contents of BUN and Cr were detected in each group. HE staining was used to observe and score pathological changes of renal tissue in rats. Protein expressions of MMP-9 and TIMP-1 in renal tissue of rats were determined by immunohistochemistry. RESULTS:Compared with sham operation group,the contents of β2-MG and NAG in urine,serum contents of BUN and Cr were increased significantly in model group(P<0.05),and pathological score and protein expressions of MMP-9 and TIMP-1 were also increased significantly(P<0.01). Compared with model group,protein expression of MMP-9 in renal tissue were increased continuously in polydatin group and benazepril group(P<0.01);other indexes were decreased significantly(P<0.05 or P<0.01),and there was no statistical significance in each index between polydatin group and benazepril group(P>0.05). CONCLUSIONS:Polydatin can delay renal fibrosis,the mechanism of which may be associated with up-regulating protein expression of MMP-9,down-regulating protein expression of TIMP-1 and increasing the ratio of MMP-9/TIMP-1.
