Content Determination of 6 Active Components in Morus alba by HPLC
10.6039/j.issn.1001-0408.2018.07.11
- VernacularTitle:HPLC法同时测定桑白皮中6种活性成分的含量
- Author:
Zhiyong CHEN
1
;
Maixia MENG
;
Yuanyuan YANG
;
Xia DU
;
Zhifeng YANG
;
Hui REN
Author Information
1. 陕西省中医药研究院
- Keywords:
Morus alba;
HPLC;
Neochlorogenic acid;
Mulberroside A;
Chlorogenic acid;
Astragalin;
Sanggenon C;
Morusin;
Content determination
- From:
China Pharmacy
2018;29(7):911-914
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the method for the simultaneous determination of content of 6 active components as neochlorogenic acid,mulberroside A,chlorogenic acid,astragalin,sanggenon C and morusin in Morus alba,and to provide reference for improving quality control standard of M. alba. METHODS:HPLC method was adopted. The determination was performed on Agilent 5 TC-C18with mobile phase consisted of acetonitrile-0.1% formic acid(gradient elution)at the flow rate of 1 mL/min. The detection wavelength of 280 nm. RESULTS:The mass concentration linear range of neochlorogenic acid, mulberroside A,chlorogenic acid,astragalin,sanggenon C and morusin were 0.001 06-0.042 4,0.001 67-0.066 8,0.007 95-0.318, 0.001 65-0.066 0,0.005 00-0.200 and 0.001 24-0.049 6 mg/mL,respectively(all r≥0.999 6);the limits of quantitation were 0.11, 0.14,0.81,0.17,0.45 and 0.12 μg/mL,respectively;the limits of detection were 0.04,0.05,0.41,0.07,0.18 and 0.04 μg/mL, respectively;RSDs of precision test were 0.26%,0.31%,0.24%,0.27%,0.36% and 0.44%(n=6),respectively;RSDs of stability test were 0.68%,0.54%,0.62%,0.53%,0.41% and 0.73%(n=6),respectively;average method recovery rates were 99.1%,98.8%,98.8%,98.4%,98.5% and 99.9%(RSDs were 0.5%-1.5%,n=9),respectively. CONCLUSIONS:The method is simple,accurate,and can be used for simultaneous determination of 6 active components in M.alba.
