Preparation of Argininate Betulinic Acid and Its Effect of the Proliferation of Triple-negative Human Breast Cancer Cell MDA-MB-231
10.6039/j.issn.1001-0408.2018.07.10
- VernacularTitle:精氨白桦脂酸的制备及其对三阴性人乳腺癌细胞MDA-MB-231增殖的影响
- Author:
Lijuan ZHANG
1
;
Yonghong LIU
;
Tongnan GU
;
Jialiang ZHONG
Author Information
1. 首都医科大学燕京医学院基础医学学系
- Keywords:
Argininate betulinic acid;
Characterization;
Solubilization;
Triple-negative human breast cancer cell MDA-MB-231;
Proliferation inhibition
- From:
China Pharmacy
2018;29(7):906-910
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To prepare argininate betulinic acid,and to investigate the effect of the proliferation of triple-negative human breast cancer cell MDA-MB-231. METHODS:By using argininate as the solubilization carrier,argininate betulinic acid was prepared by co-grinding equal molar ratio of betulinic acid and argininate. The argininate betulinic acid was characterized with powder X-ray diffractometry,infrared spectroscopy and differential scanning calorimetry. The solubility of betulinic acid and argininate betulinic acid were compared. MTT method was used to assay the effects of 15,30,60,120 μ g/mL betulinic acid, argininate betulinic acid and 5-FU on the proliferation of MDA-MB-231 cell. RESULTS:Prepared argininate betulinic acid was a new phase which was different from the physical mixing of argininate and betulinic acid,among which carboxyl group of betulinic acid and amino group of argininate formed as a salt,and the salt had no obvious melting peak. Betulinic acid was almost insoluble in water. The solubility of betulinic acid in argininate betulinic acid aqueous solution was 50.72 μg/mL. Compared with betulinic acid,the inhibitory rate of argininate betulinic acid on the growth of MDA-MB-231 cell was increased significantly(P<0.05), there was no statistical significance between its effect and 5-FU(P>0.05). CONCLUSIONS:Argininate betulinic acid with good solubility is prepared successfully,and can inhibit the proliferation of MDA-MB-231 cell.
