Effects of Modified Rhizoma Alismatis Decoction on the Expression of AQP8 in Liver Tissue of Hyperlipidemia Model Rats
10.6039/j.issn.1001-0408.2018.05.19
- VernacularTitle:泽泻汤加味方对高脂血症模型大鼠肝组织中水通道蛋白8的影响
- Author:
Muqing ZHANG
1
;
Xue HAN
;
Siming DUAN
;
Yixin ZHANG
;
Yafen WANG
;
Nabo ZHANG
;
Yanrui WANG
Author Information
1. 河北省中医院心血管内科
- Keywords:
Modified Rhizoma Alismatis decoction;
Hyperlipidemia;
Aquaporin 8;
Rat
- From:
China Pharmacy
2018;29(5):651-655
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To observe the effects of Modified Rhizoma Alismatis decoction on the expression of aquaporin 8 (AQP8) in liver tissue of hyperlipemia model rats, and to investigate the mechanism of preventing and treating hyperlipemia.METHODS: Total of 60 rats were randomly divided into blank control group (distilled water), model group, positive control group (simvastatin 1. 89 mg/kg) and modified Rhizoma Alismatis decoction high-dose, medium-dose and low-dose groups (29. 56, 14. 78, 7. 39 g/kg, calculated by crude drug), with 10 rats in each group. Those groups were given high-fat diet to induce hyperlipemia model and given relevant medicine intragastrically once a day for consecutive 5 weeks except that blank control group was given normal diet. After administration, the serum contents of TG, TC, HDL-C and LDL-C in rats were detected, and the pathomorphology changes of liver tissue were observed; the mRNA and protein expression of AQP8 in liver tissue were detected. RESULTS: Compared blank control group, the serum contents of TG, TC and LDL-C in model group were increased significantly (P<0. 05 or P<0. 01),while the serum content of HDL-C was decreased significantly (P<0. 01); pathological changes were found in liver tissue, such as irregular cell arrangement and hepatic sinusoidal hyperemia and edema; mRNA and protein expression of AQP8 in liver tissue were increased significantly (P<0. 01). Compared with model group, above indexes of treatment groups were improved significantly (P<0. 05 or P<0. 01); the structure of liver tissue tended to be normal and the fatty degeneration was obviously alleviated. CONCLUSIONS: Modified Rhizoma Alismatis decoction can regulate the mRNA and protein expression of AQP8 in liver tissue so as to play the effects on the prevention and treatment of hyperlipidemia.
