Preparation and Quality Evaluation of Zedoary Turmeric Oil Compound Liposomes
10.6039/j.issn.1001-0408.2018.05.10
- VernacularTitle:复方莪术油脂质体的制备及质量评价
- Author:
Ji CHEN
1
;
Xiaoqian ZHAO
;
Shanshan HU
;
Yanqiao MA
;
Yan LIU
;
Danfeng TAN
;
Zehui CHEN
;
Yingbiao TIAN
Author Information
1. 遵义医学院附属医院药剂科
- Keywords:
Zedoary turmeric oil;
Tretinoin;
Liposomes;
Encapsulation efficiency;
Drug-loading amount;
Preparation technology;
Quality evaluation
- From:
China Pharmacy
2018;29(5):615-620
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To prepare zedoary turmeric oil compound liposomes (ZTOC-LPS) and evaluate its quality.METHODS: The preparation method of liposome, the addition amount of soybean phosphatidylcholine (SPC), ratio of SPC to cholesterol (CH) in lipid, drug-lipid ratio of zedoary turmeric oil (ZTO), drug-lipid ratio of tretinoin in formulation, and water bath temperature were screened using encapsulation efficiency and drug-loading amount of ZTO (represented by germacrone) and tretinoin as investigation indexes. Quality evaluation and primary stability investigation were conducted for liposomes prepared by optimal preparation technology. RESULTS: The optimal preparation method was ethanol injection method; The optimal preparation technology were SPC 4 mg in 1 mL lipid, the mass ratio of SPC to CH 3:1, the ratio of ZTO to lipid 1:9, the ratio of tretinoin to lipid 1:70, water bath temperature of 55 ℃. Encapsulation efficiencies of ZTO and tretinoin were (64. 63 ± 1. 00)％ and (90. 33 土 0. 72)％ in 3 batches of ZTOC-LPS, respectively. Drug-loading amount of ZTO and tretinoin were (9. 09 ± 0. 14)％ and (1. 43 ± 0. 02)％, respectively. Particle size was (257. 41 ± 7. 58) nm, Zeta potential was (-38. 77 ± 0. 81) mV,PDI was 0. 10 ± 0. 04; the results of centrifugal acceleration test showed that the liposomes had good physical stability. No obvious change was found in each investigation index of ZTOC-LPS that stored at (4 ± 2) ℃ for 1 month. CONCLUSIONS: Established preparation technology is simple and feasible, the quality of the prepared ZTOC-LPS conforms to the requirements, and it can provide reference for the following research of ZTOC-LPS.
