Optimization of the Extraction Technology of Total Flavonoids from the Roots and Stems of Angelica sinensis
10.6039/j.issn.1001-0408.2018.03.18
- VernacularTitle:当归根、茎中总黄酮的提取工艺优化
- Author:
Xudong LUO
1
;
Chengyi LI
;
Xu LI
;
Zhengze QIANG
;
Xiaoli FENG
;
Mingwei WANG
;
Shuo LI
;
Yan WANG
Author Information
1. 甘肃中医药大学药学院
- Keywords:
Angelica sinensis;
Roots;
Stems;
Total flavonoids;
Single factor test;
Box-Behnken design;
Response methodology;
Reflux extraction;
Technology optimization
- From:
China Pharmacy
2018;29(3):364-368
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To optimize reflux extraction technology of total flavonoids from the roots and stems of Angelica sinensis. METHODS: The reflux extraction technology of total flavonoids from the roots and stems of A. sinensis was optimized by Box-Behnken design-response methodology based on single factor test using volume fraction of extraction solvent ethanol, solide-liquid ration, extraction time, extraction times as investigation factors, the content of total flavonoids in extract as evaluation index. RESULTS: The optimal extraction technology of total flavonoids from the roots and stems of A. sinensis was that volume fractions of ethanol were 70% and 50%; solid-liquid ratios were 1: 40 and 1: 30; extraction time were 1. 3 h and 1. 7 h; The number of extraction times is two times. In verification test, the contents of total flavonoids were 7. 253 6, 25. 144 1 mg/g (RSD= 1. 57%, 1. 49%, n = 3); relative errors of those to predicted value (6. 942 8, 25. 703 5 mg/g) were 4. 28%, 2. 24%. CONCLUSIONS: Optimized extraction technology for total flavonoids from the roots and stems of A. sinensis is simple, reproducible and predictable.