Simultaneous Determination of 7 Components in Siwei Jianghuang Decoction Powder by HPLC
10.6039/j.issn.1001-0408.2018.01.08
- VernacularTitle:HPLC法同时测定四味姜黄汤散中7种成分的含量
- Author:
Ya ZHAO
1
;
Hui FENG
;
Zhen ZHOU
;
Lu HAO
;
Banghua ZHOU
;
Xianrong LAI
Author Information
1. 成都中医药大学民族医药学院
- Keywords:
Siwei jianghuang decoction powder;
HPLC;
Gallic acid;
Magnoflorine;
Ellagic acid;
Jatrorrhizine hydrochloride;
Palmatine hydrochloride;
Berberine hydrochloride;
Curcumin;
Content determination
- From:
China Pharmacy
2018;29(1):29-33
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of gallic acid,magnoflorine,ellagic acid,jatrorrhizine hydrochloride,palmatine hydrochloride,berberine hydrochloride and curcumin in Siwei jianghuang decoction powder.METHODS:HPLC method was adopted.The determination was performed on Capcell Pak C18-MG Ⅱ column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid (gradient elution) at the flow rate of 0.8 mL/min.The detection wavelengths were 270 nm (0-60 min,gallic acid,magnoflorine,ellagic acid,jatrorrhizine hydrochloride,palmatine hydrochloride,berberine hydrochloride) and 428 nm (60-70 min,curcumin).The column temperature was set at 30 ℃,and sample size was 10 μL.RESULTS:The linear ranges of gallic acid,magnoflorine,ellagic acid,jatrorrhizine hydrochloride,palmatine hydrochloride,berberine hydrochloride and curcumin were 0.249 6-1.497 6,0.284 0-1.704 0,0.075 6-0.453 6,0.015 9-0.095 9,0.023 6-0.141 6,0.098 2-0.589 0 and 0.060 4-0.362 4 μtg (r≥0.999 8).The limits of detection were 6.24,4.73,7.56,2.36,3.20,6.54,6.04 ng,and the limits of quantitation were 17.47,16.08,20.86,7.31,10.24,19.62,19.32 ng,respectively.RSDs of precision,stability (12 h),reproducibility tests were lower than 2.0％ (n=6).The recoveries were 95.45％-103.47％ (RSD=0.86％-1.98％,n=9).CONCLUSIONS:Established method is simple,accurate,reliable and suitable for simultaneous determination of 7 components such as gallic acid in Siwei jianghuang decoction powder.
