Simultaneous Determination of Liquiritin and Glycyrrhizic Acid in Fuzi Lizhong Pills (Condensed Pills) by HPLC
10.6039/j.issn.1001-0408.2017.36.28
- VernacularTitle:HPLC法同时测定附子理中丸(浓缩丸)中甘草苷和甘草酸的含量
- Author:
Dongwei FANG
1
;
Jiaqing PENG
Author Information
1. 鄂州市食品药品检验检测中心
- Keywords:
Fuzi lizhong pills (condensed pills);
HPLC;
Liquiritin;
Glycyrrhizic acid;
Content determination
- From:
China Pharmacy
2017;28(36):5147-5149
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the method for simultaneous determination of liquiritin and glycyrrhizic acid in Fuzi lizhong pills (condensed pills).METHODS:HPLC method was adopted.The determination was performed on WondaSil C18 column with mobile phase consisted of acetonitfile-0.05％ phosphate solution (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 237 nm,and column temperature was 35 ℃.The sample size was 10 μL.RESULTS:The linear range ofliquiritin and glycyrrhizic acid were 9.68-96.8 μg/mL(r=0.999 1)and 14.08-140.8 μg/mL(r=0.999 2).The limits of quantitation were 0.2,0.3 μg/mL,and the limits of detection were 0.1,0.01 μg/mL.RSDs of precision,stability and reproducibility tests were all lower than 2.0％.The average recoveries were 98.9％-101.2％ (RSD=0.62％,n=9),98.6％-101.5％ (RSD=1.06％,n=9).CONCLUSIONS:The method is simple and accurate,and can be used for simultaneous determination of liquiritin and glycyrrhizic acid in Fuzi lizhong pills (condensed pills).
