Study on the Differences of Inorganic Elements in Different Parts of Saururus chinensis
10.6039/j.issn.1001-0408.2017.36.24
- VernacularTitle:三白草药材不同部位中微量元素的差异研究
- Author:
Hongjiang CHEN
1
;
Jianan LIU
;
Shaojun CHEN
;
Ping XUE
Author Information
1. 浙江医药高等专科学校中药学院
- Keywords:
Saururus chinensis;
ICP-MS;
Trace elements;
Principal component analysis;
PLS-DA
- From:
China Pharmacy
2017;28(36):5131-5135
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the differences of inorganic elements in different parts of Saururus chinensis.METHODS:The samples were treated with microwave digestion.The contents of 25 inorganic elements were determined by inductively coupled plasma mass spectrometry (ICP-MS) method:RF power of 1 200 W,cooling gas flow of 14 L/min,auxiliary gas flow of 1.0 L/ min,atomizing air flow of 0.9 L/min,He as collision gas with flow rate of 4.8 mL/min,sampling cone aperture of 1.1 mm,skimmer cone aperture of 0.9 mm,super skimmer cone aperture of 1.0 mm,integral time of 1 s,delay time of 40 s,single hop as scanning mode,repeated for 3 times.The data of principal component analysis and partial least squares discriminant analysis were analyzed with the SIMCA-P 13.0 statistical software.RESULTS:The linear ranges of lithium (Li),beryllium (Be),boron (B),magnesium (Mg),aluminum (Al),titanium (Ti),vanadium (V),chromium (Cr),manganese (Mn),iron (Fe),cobalt (Co),nickel (Ni),copper (Cu),zinc (Zn),gallium (Ga),arsenic (As),cobalt (Sr),cadmium (Cd),tin (Sn),antimony (Sb),barium (Ba),mercury (Hg),thallium (Ti),lead (Pb) and bismuth (Bi) were 0.05-5.0 μg/L(r=0.999 9),0.05-5.0 μg/L(r=0.999 8),0.1-10.0 μg/L(r=0.999 5),1.0-100.0 μg/L(r=0.999 5),1.00-100.0 μg/L(r=0.999 3),0.10-10.0 μg/L(r=0.999 9),0.05-5.0 μg/L (r=0.999 7),0.05-5.0 μg/L(r=0.999 5),0.10-10.0 μg/L(r=0.999 8),1.00-100.0 μg/L(r=0.999 4),0.05-5.0 μg/L(r=0.999 9),0.05-5.0 μg/L(r=0.999 7),0.05-5.0 μg/L(r=0.999 5),0.05-5.0 μg/L(r=0.999 8),0.05-5.0 μg/L(r=0.999 6),0.05-5.0 μg/L(r=0.999 8),0.05-5.0 μg/L(r=0.999 9),0.05-5.0 μg/L(r=0.999 3),0.05-5.0 μg/L(r=0.999 4),0.05-5.0 μg/L(r=0.999 8),0.1-10.0 μg/L(r=0.999 6),0.05-5.0 μg/L(r=0.999 6),0.05-5.0 μg/L(r=0.999 6),0.05-5.0 μg/L(r=0.999 7),0.05-5.0 μg/L(r=0.999 4),respectively.The detection limits were no higher than 13.2 ng/L.RSDs of precision,stability and reproducibility tests were all lower than 5.12％.The recoveries ranged 90.18％-123.01％ (RSD were 2.05％-5.79％,n=6).The contents of Sb,Sn,Mn,Cd,Ti,Ba,Co,As,Be,Ga,Ni were higher in aerial part than in rhizome part,while the content of Cr was higher in rhizome part than in aerial part.CONCLUSIONS:Mg,Fe and A1 are abundant in aerial part and rhizome part of S.chinensis.The contents of inorganic elements are significantly different in different parts of S.chinensis.
