Study on HPLC Fingerprint of Miao Medicine Ardisia crenata
10.6039/j.issn.1001-0408.2017.30.29
- VernacularTitle:苗药朱砂根的HPLC指纹图谱研究
- Author:
Xu SUN
1
;
Chengfen YAO
;
Sihong FU
;
Zaipeng GONG
;
Ting LIU
;
Chang YANG
;
Jun ZHA
;
Yongjun LI
Author Information
1. 贵州省药物制剂重点实验室
- Keywords:
Ardisia crenata;
HPLC;
Fingerprint;
Cluster analysis
- From:
China Pharmacy
2017;28(30):4285-4288
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish HPLC fingerprints of Miao medicine Ardisia crenata.METHODS:HPLC method was adopted.The determination was performed on Diamonsil C18 column with mobile phase consiste of methanol-water (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was 220 nm,and column temperature was maintained at 30 ℃.The sample size was 10 μL.Using 11-O-(3',4',5'-three-o-galloylhyperin)-bergeninum as reference,HPLC fingerprints of 16 batches of samples were determined.Common identification and similarity evaluation were performed by using TCM Chromatographic Fingerprint Similarity Evaluation Software (2012 edition).Cluster analysis of fmgerprrints was conducted.RESULTS:There were 6 common peaks in HPLC fingerprints of 16 batches of samples.The similarity among 8 batches was more than 0.9.The 16 batches of samples could be clustered into 4 categories.CONCLUSIONS:Established fingerprints can provide reference for identification and quality evaluation ofA.crenata.
