Study on HPLC Fingerprint of Qingbi Granules
10.6039/j.issn.1001-0408.2017.30.28
- VernacularTitle:清痹颗粒的HPLC指纹图谱研究
- Author:
Xuejie BAI
1
;
Miao YU
;
Xiuhua LIU
;
Xueyuan BAI
;
Qingjie LI
Author Information
1. 长春医学高等专科学校药学系
- Keywords:
Qingbi granules;
Fingerprint;
HPLC
- From:
China Pharmacy
2017;28(30):4282-4285
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish HPLC fingerprint of Qingbi granules.METHODS:HPLC method was adopted.The determination was performed on Diamonsil C18 column with mobile phase consisted of methanol-0.3％ phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 260 nm,and column temperature was 25 ℃.The sample size was 10 μL.Using baicalin as reference,HPLC chromatograms of 10 batches of samples were determined.Common peak identification and similarity evaluation were performed by using TCM Chromatographic Fingerprint Similarity Evaluation Software (2.0 edition).RESULTS:There were 29 common peaks in HPLC chromatograms of 10 batches of samples.The similarity among the 10 batches was more than 0.90.After validation,HPLC chromatograms of 10 batches of samples were in line with control fingerprints.CONCLUSIONS:Established fingerprints can provide reference for identification and quality evaluation of Qingbi granules.
