Simultaneous Determination of Eugenol and Thymol in Mummification Pastes by HPLC

VernacularTitle:HPLC法同时测定干髓糊剂中丁香酚和麝香草酚的含量
Author: Weiyu CAO ¹ ; Wenjuan HE ; Wan LEI ; Xiaojuan WANG
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1. 军事口腔医学国家重点实验室/国家口腔疾病临床医学研究中心/陕西省口腔生物工程技术研究中心/第四军医大学口腔医院药剂科
Keywords: HPLC; Mummification pastes; Eugenol; Thymol; Content determination
From: China Pharmacy 2017;28(30):4268-4271
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To establish a method for simultaneous determination of eugenol and thymol in mummification pastes.METHODS:HPLC method was adopted.The determination was performed on Wonda Cract C18 column with mobile phase consisted of methanol-0.2％ phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was 274 nm,and column temperature was set at 25 ℃.The sample size was 10 μL.RESULTS:The linear ranges of eugenol and thymol were 0.02-0.19 mg/mL (r=0.999 7) and 0.03-0.30 mg/mL (r=0.999 6).RSDs of precision,stability and reproducibility tests were all lower than 2.0％.The recoveries were 95.85％-99.84％(RSD=1.11％,n=9) and 95.81％-100.91％(RSD=1.59％,n=9).CONCLUSIONS:The method is simple and accurate,and can be applied to simultaneous determination of eugenol and thymol in mummification pastes.