Simultaneous Determination of 7 Components in Tanreqing Capsules by HPLC
10.6039/j.issn.1001-0408.2017.30.21
- VernacularTitle:HPLC法同时测定痰热清胶囊中7种成分的含量
- Author:
Zhenhua ZHANG
1
;
Pingping ZHONG
;
Ying XU
Author Information
1. 上海凯宝药业股份有限公司
- Keywords:
Tanreqing capsules;
Chlorogenic acid;
Isoforsythiaside A;
Forsythiaside A;
Isochlorogenic acid;
Baicalin;
HPLC;
Content determination
- From:
China Pharmacy
2017;28(30):4256-4260
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of 7 components in Tanreqing capsules.METHODS:HPLC method was adopted.The determination was performed on Ultimate XB C18 column with mobile phase consisted of acetonitrile-0.2％ formic acid (gradient elution) at a flow rate of 0.8 mL/min.The detection wavelength was set at 325 nm,and column temperature was 35 ℃.The sample size was 10 μL.RESULTS:The linear ranges of chlorogenic acid,isoforsythiaside A,forsythiaside A,isochlorogenic acid B,isochlorogenic acid A,isochlorogenic acid C and baicalin were 0.025-0.5 μg(r=0.999 6),0.025-0.5 μg (r=0.999 7),0.050-1.0 μg (r=0.999 9),0.025-0.5 μg((r=0.999 7),0.025-0.5 μg ((r=0.999 6),0.025-0.5 μg (r=0.999 6),0.750-1.5 μg(r=0.999 9),respectively.The limit of quantitation was no more than 1.5 ng,and the limit of detection was 0.5 ng.RSDs of precision,stability and reproducibility tests were all lower than 3.0％.The recoveries were 95.28％-106.30％ (RSD ranged 0.97％-2.14％,n=9).CONCLUSIONS:The method is simple,precise,stable and reproducible,and can be used for simultaneous determination of 7 components in Tanreqing capsules.
