Rapid Determination of Multi-maker Ingredients in Salvia miltiorrhiza by Near Infrared Diffused Reflection Spectroscopy
10.6039/j.issn.1001-0408.2017.30.19
- VernacularTitle:近红外漫反射光谱法快速测定丹参药材中多指标成分的含量
- Author:
Zhen LI
1
;
Lihong ZHOU
;
Gongjun YANG
;
Zhengliang YE
Author Information
1. 中国药科大学药物分析教研室
- Keywords:
Near infrared diffuse reflectance spectroscopy;
Salviae miltiorrhizae;
Phenolic acids;
Tanshinone;
Rapid determination;
Content
- From:
China Pharmacy
2017;28(30):4247-4251
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the method for rapid determination of nuezhenoside in Salvia miltiorrhiza.METHODS:The contents of salvianolic acid B,rosmarinic acid,lithospermic acid and tanshinone (tanshinone Ⅱ A+tanshinone Ⅰ +cryptotanshinone) were determined by HPLC (as reference value).Quantitative model for the contents of above components was established by partial least square (PLS)-NIR spectra.According to the reference value,143 samples were collected and the spectrum was pretreated by first-order derivative.The optimal range of wave band for salvianolic acid B,rosmarinic acid,oxalic acid and tanshinone were 6 773.98-3 981.12,6 670.85-3 996.54,8 544.66-3 936.28,8 188.06-3 875.31 cm-1.RESULTS:The methodology for the content determination of salvianolic acid B,rosmarinic acid,oxalic acid and tanshinone were in line with the requirement.The coefficient (R2) of internal cross validation for quantitative correction model of salvianolic acid B,rosmarinic acid,oxalic acid and tanshinone were 0.919 0,0.832 2,0.821 5,0.925 6.The deviation of corrected mean square roots were 4.46,0.48,1.34,0.71,respectively.The R2 values of external validation were 0.852 6,0.957 3,0.819 3,0.953 1,respectively;and mean square root of prediction error (RMSEP)were 9.77,0.28,0.94,0.63,respectively.CONCLUSIONS:The method is rapid,accurate,simple and pollution-free,and can be used for rapid determination of multi-maker ingredients in S.miltiorrhiza.
