The Role of AMPKα2 in High-intensity Interval Training-activated Autophagy in the Skeletal Muscle of C57BL/6 Mice
- VernacularTitle:AMPKα2在高强度间歇运动激活小鼠骨骼肌细胞自噬过程中的作用
- Author:
Lingjian XIE
1
;
Shan ZHANG
;
Sujuan LIU
;
Li FU
Author Information
1. 天津医科大学基础医学院
- Keywords:
high-intensity interval training(HIIT);
BMI;
autophagy;
AMP activated protein kinase
- From:Chinese Journal of Sports Medicine
2018;37(4):309-314
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of 6-week high-intensity interval training(HIIT) on the body composition and the autophagy activation in skeletal muscle of C57BL/6 mice,and explore the underlying role of adenosine 5'-monophosphate(AMP)-activated protein kinase(AMPK)α2 in this process.Methods Thirty-six 4-week old male mice with the genotype AMPKα2+/+(n=12),AMPKα2+/-(n=12) and AMPKα2-/-(n=12),were randomly divided into a control(n=6) and an HIIT group(n=6) respectively.The mice from the HIIT group exercised on a motor-driven rodent treadmill for 5 days a week,lasting 6 weeks.The procedure of HIIT included the intensity of 85％ VO2max(20m/min) for 2 min/day and 50％ VO2max(8m/min) for 1 min/day with 12 cycles alternately.The body weight,body mass index(BMI),total body water and fat mass were measured and analyzed after the 6-week exercise.The protein expression of AMPKα2,pAMPK-Thr172,LC3Ⅱ/Ⅰ,Beclin1 and p62 in the skeletal muscle tissue were detected using Western blotting.Results Before exercise,there was no significant difference between the mice of the same genotype in the body weight.However,a significant decrease was observed in the body weight of all the HIIT mice,and BMI of the AMPKα2+/+ and AMPKα2+/-mice undergoing HIIT.There were no significant differences in the total body water between the control and HIIT groups of the same genotype after the intervention.There was significant decrease in the body fat mass in the HIIT group of AMPKα2+/+ and AMPKα2+/-mice after the intervention,while the decrease was not significant in the HIIT AMPKα2-/-mice.The protein expression of pAMPK-Thr172,LC3Ⅱ/Ⅰ and Beclin1 increased significantly,and that of p62 decreased significantly in the skeletal muscle of the AMPK+/+ and AMPK+/-mice after HIIT intervention,but not the AMPK-/-mice.Conclusion HIIT can improve health indicators(i.e,body weight,BMI and fat mass) and enhance autophagy activation in the skeletal muscle through increasing the phosphorylation of AMPKot2.
