The Effect of Temperature-sensitive Hydrogel Platelet-richplasma Complex on the Healing of Anterior Cruciate Ligament Partial Tear in a Rat Model
10.3969/j.issn.1000-6710.2017.12.007
- VernacularTitle:温度敏感的水凝胶与富血小板血浆复合体对大鼠前交叉韧带部分损伤愈合的作用
- Author:
Yue LI
1
;
Chuen Sai FU
;
Guangping LI
;
Hua FENG
Author Information
1. 北京积水潭医院运动医学科 北京100035
- Keywords:
anterior cruciate ligament;
ACL;
Hydrogel;
partial tear;
PRP;
histology;
biomechanics
- From:Chinese Journal of Sports Medicine
2017;36(12):1072-1075,1111
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of the temperature-sensitive hydrogel and platelet-rich plasma(PRP)complex on the healing of anterior cruciate ligament (ACL)partial tear in a rat model.Method PRP was processed according to an established method and then mixed with poly(ethylene glycol)monomethyl ether and poly(lactic-co-glycolic acid)(mPEG-PLGA).A total of 110 right knees of Sprague-Dawley male rats (12-week old)were included and randomly divided into a healthy control group (n=10),a lesion control group(n=60)and a study group(n=40).The saline and mPEG-PLGA-PRP complex was applied at the lesion site respectively.Samples were harvested 0,2 weeks and 6 weeks post-operatively and the histological and biomechanical changes were observed and compared among the 3 groups.Results Histologically,at 6 weeks after surgery,the torn ACL of the study group had been partially healed,with decreased number of inflammatory cells and new fibrous tissues and micro-vessels appearing.The ligament maturity index revealed a significantly higher score in the study group than the lesion control group(20.6 ± 4.9 vs.4.7 ± 1.0,P＜0.01).Biomechanically,at 6 weeks after surgery,the tensile strength of the study group was significantly higher than the contro] groups(52.7 ± 11.2 vs.30.3 ± 8.8,P＜0.05).Conclusion At six weeks after surgery,the mPEG-PLGA-PRP complex can enhance the healing of ACL partial tear,ahhough the ligament was not recovered to the norma] state.
