Effects of Acute Exercises of Different Intensities on the FNDC5 and UCP-1 Expression in Epididymal WAT of Rats
10.3969/j.issn.1000-6710.2017.12.004
- VernacularTitle:不同强度一次性运动对大鼠睾周白色脂肪FNDC5、UCP-1表达量的影响
- Author:
Rui XU
1
;
Yi YAN
;
Minhao XIE
Author Information
1. 北京体育大学运动人体科学学院 北京100084
- Keywords:
acute exercises;
browning;
UCP-1;
FNDC5
- From:
Chinese Journal of Sports Medicine
2017;36(12):1052-1058
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of acute exercises of different intensities on the "browning" of epididymal white adipose tissue(WAT) in rats.Methods Thirty Sprague-Dawley male rats were randomly divided into a control group(C,n=6) and an exercises group(E,n=24).Rats in group E were further randomly divided into a moderate intensity exercise (EM,n=12,V=15 m/min) group and a high intensity intermittent exercise(EH,n=12,V=35 m/min,6min's exercises followed by 5 min's rest,repeating 3 times) group.Right after and 6 hours after the acute exercises,the epididymal WAT was taken,and the fibronectin type Ⅲ domain-containing 5 protein(FNDC5) and the uncoupling protein 1(UCP-1) mRNA were detected using RT-qPCR,while the level of FNDC5 and UCP-1 protein was evaluated using Western Blotting.Results Right after the acute exercises,compared with group C,the level of FNDC5 mRNA in group EH increased significantly (P＜0.01),while that of UCP-1 mRNA in group E decreased immediately(P＜0.05) but increased significantly six hours later(P＜0.05).Compared with group C,the level of FNDC5 in groups EM and EH tended to rise,and that of group EM increased significantly(P＜0.05).Compared with group C,the level of UCP-1 in group EM and EH had the tendency to rise,and it increased significantly immediately after exercises in group EH (P＜0.05).Conclusion The acute exercise at different intensities can promote the level of FNDC5 and UCP-1 in epididymal white adipose tissue,"browning" and heat production from fat issues.The level of UCP-1 mRNA increased significantly at 6 hours after exercises,while that of FNDC5 mRNA increased most immediately after the high-intensity intermittent exercises.
