Expression and purification of human placental growth factor 2 using the Pichia pastoris expression system
10.11665/j.issn.1000-5048.20180318
- VernacularTitle:人胎盘生长因子2在毕赤酵母中的表达和纯化
- Author:
Xiaoqing LIANG
1
;
Pengfei YAN
;
Yu LIU
Author Information
1. 东南大学附属中大医院
- Keywords:
placental growth factor 2 (PIGF-2);
Pichia pastoris;
purify
- From:
Journal of China Pharmaceutical University
2018;49(3):376-380
- CountryChina
- Language:Chinese
-
Abstract:
To prepare the human placental growth factor 2 (PIGF-2 ),human PIGF-2 gene was cloned into pPIC9K vector to construct the recombinant pPIC9K-PIGF-2 vector. Linearized recombinant pPIC9K-PIGF-2 was transformed into Pichia pastoris by electroporation. YPD-Geneticin plate was used to screen geneticin hyper-resistant colonies. The positive colonies were verified by PCR. Results of SDS-PAGE and Western blot showed that recom-binant human PIGF-2 was expressed after being induced by methanol. Using its characteristic heparin binding, recombinant human PIGF-2 was successfully purified by heparin affinity column chromatography.
