lmmunogenicity of HER2 vaccine containing p-nitrophenylalanine
10.11665/j.issn.1000-5048.20180317
- VernacularTitle:含对硝基苯丙氨酸HER2疫苗的免疫原性研究
- Author:
Yu HE
1
;
Hong TIAN
;
Xin DAI
;
Wenbing YAO
;
Xiangdong GAO
Author Information
1. 中国药科大学生命科学与技术学院
- Keywords:
HER2;
p-nitrophenylalanine;
immunogenicity
- From:
Journal of China Pharmaceutical University
2018;49(3):369-375
- CountryChina
- Language:Chinese
-
Abstract:
In order to delete the immune tolerance of self-protein HER2 and produce an efficient immune response,the genetic code expansion technique was employed to introduce the immunogenic amino acid p-nitro-phenylalanine into the position 5 ,26 and 79 of the HER2 fragment and HER2 mutants containing p-nitropheny-lalanine were obtained.Prototype and mutant HER2 molecules with a purity of more than 95% were obtained through a nickel affinity column.Immunogenicity analysis of the HER2 vaccine showed that pNO2Phe79HER2 was able to produce high titer cross-reactive antibodies in C57BL/6 mice and that the mouse antisera could recognize SKBR-3 cells with intact HER2 receptor extracellular domain and cleave HER2 highly expressed HER2 +B16F10 cells through ADCC effect.These findings suggested that pNO2Phe79HER2 which incorporates p-nitro-phenylalanine could be used as a tumor vaccine candidate targeting HER2.
