Mechanisms of miR-29a in migration and invasion of breast cancer MCF-7 cells in vitro
10.11665/j.issn.1000-5048.20180314
- VernacularTitle:miR-29a促进人乳腺癌MCF-7细胞体外迁移和侵袭的机制
- Author:
You WU
1
;
Tingting TANG
;
Qinhua ZHU
;
Liang JIN
;
Yi PAN
Author Information
1. 中国药科大学生命科学与技术学院,南京210009
- Keywords:
miR-29a;
breast cancer;
MCF-7 cells;
cancer metastasis;
migration;
invasion;
HBP1 protein
- From:
Journal of China Pharmaceutical University
2018;49(3):348-353
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the effect and mechanisms of miR-29a in migration and inva-sion of human breast cancer MCF-7 cells in vitro. MCF-7 cells were treated with miR-29a mimic or miR-29a inhibitor to up-regulate/down-regulate the expression level of miR-29a. Wound-healing assay and transwell chamber were employed to determine cell migration and invasion in vitro. The target gene of miR-29a was predic-ted with the Targetscan7. 1 database and verified through luciferase reporter method. The effects of miR-29a on the expression of the potential target were detected by Western blot and real-time PCR. Results showed that in vitro migration and invasion ability of MCF-7 cells was increased significantly by miR-29a,which could target HBP1 in the 3′-UTR region. The protein expression of HBP1 was decreased by miR-29a overexpression. However, the alteration of miR-29a had no significant effect on the expression of HBP1 mRNA. The results validated that miR-29a,highly expressed in breast cancer,could down-regulate HBP1 ,which in turn promotes migration and invasion ability of breast cancer cells,thus promoting breast cancer metastasis.
