Effects of various autophagy modulators on the expression of autophagic markers LC3ll and p62
10.11665/j.issn.1000-5048.20180313
- VernacularTitle:多种细胞自噬调节剂对自噬标记物LC3Ⅱ及p62表达的影响
- Author:
Yanming WEI
1
;
Jinhong REN
;
Zhihua LUAN
;
Yonghui WANG
Author Information
1. 山西中医药大学制药与食品工程学院
- Keywords:
autophagy activator;
autophagy inhibitor;
LC3Ⅱ;
p62
- From:
Journal of China Pharmaceutical University
2018;49(3):341-347
- CountryChina
- Language:Chinese
-
Abstract:
Western blotting of autophagic markers LC3Ⅱ and p62 are widely used for estimating autophagic activity. To compare the regulation of various autophagy modulators on LC3Ⅱ and p62,HEK293 cells were treated separately with mTOR-dependent autophagy activator rapamycin or -independent autophagy activators trehalose, and autophagy inhibitors including 3-methyladenine (3-MA),bafilomycin A1 or E64d and pepstatin A that inhi-bited the initiation of autophagy,the fusion of autophagosome and lysosome,and the activities of lysosomal enzymes accordingly,and then LC3Ⅱ and p62 levels were assessed. Western blot results demonstrated that rapam-ycin enhanced the conversion of LC3I to LC3Ⅱ,promoted the degradation of p62 simultaneously,while trehalose merely increased the expression of LC3Ⅱ with no influence on the p62 level. Moreover,inhibition of autophagy commonly led to accumulation of LC3Ⅱ as well as blockage of p62 degradation in a concentration- and time-dependent manner. These results indicate that obvious differences exist in the regulation of LC3Ⅱ and p62 by various modulators although both are autophagic markers.
