Effects and mechanism ofandrographolide on diabetic nephropathy mice
10.11665/j.issn.1000 -5048.20180114
- VernacularTitle:穿心莲内酯对糖尿病肾病小鼠的治疗作用及机制
- Author:
Mengshi LI
1
;
Teng WU
;
Jinghua CAO
;
Yajun HE
;
Cuiling QI
;
Lijing WANG
Author Information
1. 广东药科大学基础学院血管生物学研究所
- Keywords:
andrographolide;
diabetic nephropathy;
Leprdb/dbmice;
mesangial cells
- From:
Journal of China Pharmaceutical University
2018;49(1):97-101
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects and mechanism of andrographolide(Andro)on type II diabetic nephropathy in Leprdb/dbmice,levels of urinary protein,serum creatinine and blood urea nitrogen after 24 h of Andro(1 mg/kg,ip)treatment on Leprdb/dbmice were measured by kit.The changes on renal pathology and sugar levels in diabetic mice treated by Andro were observed by HE staining and PAS staining.The expression of fibronectin (FN)and NOX-4 protein were analyzed by immunofluorescence.Results showed that levels of urinary protein, serum creatinine and blood urea nitrogen in Leprdb / dbmice after Andro treatment for 24 h were significantly lower than those of the control group. Kidney pathologic change was inhibited, and FN and NOX-4 expression were markedly decrease in the Andro group.In conclusion,Andro can significantly reduce the diabetic nephropathy in Leprdb/dbmice with spontaneous type II diabetes mellitus.
