Transcriptomic analysis of a spiramycin Ⅰ-resistant Staphylococcus aureus mutant
10.11665/j.issn.1000-5048.20170617
- VernacularTitle:螺旋霉素对金黄色葡萄球菌耐药突变体的转录组特征分析
- Author:
Jian YAO
1
;
Lei SHAO
;
Daijie CHEN
;
Yubin ZHANG
Author Information
1. 中国药科大学生命科学与技术学院生物化学教研室
- Keywords:
Staphylococcus aureus;
antibiotic resistance;
spiramycin Ⅰ;
transcriptome analyses;
arginine metabolic pathways
- From:
Journal of China Pharmaceutical University
2017;48(6):738-744
- CountryChina
- Language:Chinese
-
Abstract:
The clinical utility of macrolide antibiotics has declined due to the appearance of resistant isolates.A spiramycin Ⅰ-resistant Staphylococcus aureus ATCC29213-R was induced and isolated with increasing the concentration of spiramycin Ⅰ,which exhibits an A→C transversion at position 2089 in the 23S rRNA gene,which is first reported in the S.aureus.A RNA-seq based transcriptomic analysis was performed to understand the overall response of resistant bacteria to spiramycin Ⅰ treatment with subinhibitory dosage.Inn this study,There are a total of 322 up-regulated and 82 down-regulated genes in spiramycin Ⅰ-treated S.aureus ATCC29213-R and 426 up regulated,838 down-regulated in spiramycin Ⅰ-treated S.aureus ATCC29213,which were identified differentially expressed compared to their control with a minimum 2-fold change (Q ＜ 0.05).Interestingly,The data showed that argH and argG transcripts,in the arginine biosynthetic pathway,were decreased by 13.51-fold and 21.45-fold,respectively,compared to the control,while the expression level of three genes involved in arginine catabolism,arcA,arcC,and argF,increased by 35-fold,18.05-fold and 30.84-fold,respectively.The results revealed that spiramycin Ⅰ could trigger the up-regulation of the genes of ACME-Arc system which allows S.aureus to survive in acidic environments of human skin.This suggesed the arginine-deiminase pathway may be a potential target for treatment of the resistant S.aureus.
