Optimization of in vitro human follicular helper T cell differentiation condition
10.11665/j.issn.1000-5048.20170616
- VernacularTitle:人Tfh细胞体外诱导分化条件的研究
- Author:
Yucong CHEN
1
;
Wei GUO
;
Wen LEI
;
Yaoyao GE
;
Wenyao XUE
;
Qianwen LI
;
Xiangdong GAO
Author Information
1. 中国药科大学生命科学与技术学院分子生物学教研室
- Keywords:
follicular helper T cell;
TCR signal;
in vitro differentiation
- From:
Journal of China Pharmaceutical University
2017;48(6):733-737
- CountryChina
- Language:Chinese
-
Abstract:
In order to explore the regulation mechanisms of follicular helper T cell (Tfh Cell) differentiation,optimized conditions of in vitro induction from both peripheral blood mononuclear cells and MAC sorted Na(i)ve CD4 + T cells to human Tfh cells were developed.Induction efficiency difference of TCR signal anti-hCD3e stimulation between coated on solid phase and in soluble phase was also determined.Differentiation efficiency of CD4 + CXCR5 + ICOS+PD-1 + Tfh cell was determined by FACS while the expression level of IL-21 in cell supernatant was determined by ELISA tests.An ultimate induction condition that 5 μg/mL coated overnight anti-hCD3e stimulated na(i)ve CD4 + T cells to differentiate into Tfh at an up to 20.4％ percentage was finally determined.The optimization of in vitro induction protocol of human Tfh provided an effective examine platform for the studies on Tfh differentiation mechanisms and related pharmacology,toxicity and metabolic experiments.
