Rapid determination of six flavonoids from seabuckthorn leaves by RP-HPLC-DAD
10.11665/j.issn.1000-5048.20170610
- VernacularTitle:RP-HPLC-DAD法快速测定沙棘叶中6种黄酮成分
- Author:
Renjie HUI
1
;
Xu HE
;
Jingyi LIU
;
Jing FENG
;
Shuai ZENG
;
Bainian FENG
Author Information
1. 江南大学药学院
- Keywords:
seabuckthorn leaves;
RP-HPLC-DAD;
flavonoids;
rapid determination
- From:
Journal of China Pharmaceutical University
2017;48(6):696-700
- CountryChina
- Language:Chinese
-
Abstract:
To establish a rapid determination method of six flavonoids:catechin,rutin,myricetin,quercetin,kaempferol and isorhamnetin,from seabuckthorn leaves by RP-HPLC-DAD.The seabuckthorn leaves were first degreased by petroleum ether,extracted by ethanol,and determined by RP-HPLC-DAD.The six flavonoids were separated and eluted by a Shimadzu C18(150 mm ×2.1 mm,5 μm) column with methanol-water (0.1％ phos phoric acid) (60∶ 40) at a flow rate of 1.0 mL/min.The detection wavelength were as follow:catechin 208 nm,rutin 257 nm,myricetin 373 nm,quercetin 371 nm,kaempferol 367 nm,and isorhamnetin 371 nm,respectively.The injection volume was 20 μL.The contents of the six flavonoids were in the range of 0.47 to 30.00 μ,g/mL with good linearity.The validation of the method,including precision,stability and recovery rate,was acceptable.The established method can be used for fast determination of the content of six flavonoids in seabuckthorn leaves.
