Study on rapid recognition technique of schistosome susceptibility water bodyⅠAnimal skin making and effect assessment
10.16250/j.32.1374.2016270
- VernacularTitle:快速识别血吸虫易感性水体技术研究Ⅰ动物皮膜制作与检测效果初报
- Author:
Guo-Hua PENG
1
;
Wang-Yuan WEI
;
Ke QIAN
;
Zhu-Hua HU
;
Shi-Zhu LI
;
Jia-Gang GUO
;
Qing YU
Author Information
1. 江西省南昌市疾病预防控制中心 南昌330038
- Keywords:
Schistosoma japonicum;
Cercaria;
Bionic technique;
Sentinel mouse method
- From:
Chinese Journal of Schistosomiasis Control
2018;30(2):200-201,210
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a bionic animal skin and evaluate its detective effect of Schistosoma japonicum cercariae so as to provide a new technique for the monitoring of the schistosome susceptibility water body.Methods A bionic animal skin was made with pigskin and the experiments were carried out for evaluating its detective effects of S.japonicum cercariae in labo-ratory and simulate fields,and at the same time,the sentinel mouse method was used as the control.Results In the laborato-ry,the cercariae were found in 10-,30-,60-cercaria subgroups in the bionic animal skin group after 1 and 2 hours,but the cer-cariae were found only in the 60-cercaria subgroup in the sentinel mouse group.In the simulate fields,in the water body with 5 schistosome-infected Oncomelania hupensis snails,the cercariae were found in 2 bionic animal skin devices(2/4)and 3 bionic animal skin devices(3/4)after 2 hours and 4 hours respectively,and in the water body with 10 schistosome-infected O.hupensis snails,the cercariae were found in 4 bionic animal skin devices(4/4)and 3 bionic animal skin devices(3/4)after 2 hours and 4 hours respectively in the bionic animal skin group.In the the sentinel mouse group,in the above-mentioned water bodies(with 5 or 10 schistosome-infected O.hupensis snails),the cercariae were found in 2 sentinel mice(2/4)and 1 sentinel mice(1/4)af-ter 2 hours and 4 hours respectively;and the cercariae were found in 2 sentinel mice(2/4)and 3 sentinel mice(3/4)after 2 hours and 4 hours respectively.Conclusion The bionic animal skin device can be used to detect the S.japonicum cercariae, and its sensibility and efficiency are superior to the traditional sentinel mouse method.