Effects of glycolytic pathway on generation of regulatory T cells during Schistosoma japonicum infection
10.16250/j.32.1374.2018006
- VernacularTitle:日本血吸虫感染过程中糖酵解途径对调节性T细胞产生的影响
- Author:
Xiao-Fan WANG
1
;
Qian-Qian QI
;
Li-Na ZHANG
;
Lei XU
;
Li-Yang DONG
;
Ya-Lin LI
;
Ya-Nan PU
;
Chuan WEI
;
Sha ZHOU
;
Ji-Feng ZHU
;
Xiao-Jun CHEN
;
Chuan SU
Author Information
1. 南京医科大学病原生物学系 南京 211166
- Keywords:
Schistosoma japonicum;
Glycolytic pathway;
Glut1;
Treg cells
- From:
Chinese Journal of Schistosomiasis Control
2018;30(2):131-135
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the influence of glycolytic pathway on the proportion and numbers of regulatory T cells dur-ing Schistosoma japonicum infection. Methods A S. japonicum-infected mouse model was established,and C57/BL6 male mice infected with S.japonicum were subjected to intraperitoneal injections of with the glycolytic inhibitor 2-Deoxy-D-glucose (2DG)or PBS for 6 times,and then the cells from spleen or mesenteric lymph nodes(LNs)were isolated and analyzed by flow cytometry(FCM)to detect the percentage of Glut1+CD4+T cells and Treg cells. Results The proportions of Glut1+CD4+T cells in the spleen(43.58%±2.50% vs.21.15%±0.96%;t=8.834,P<0.01)and mesenteric LNs(38.97%±1.97% vs.28.40%± 2.11%;t=3.662,P<0.05)were higher in the normal mice than those in the infected mice,and the percentages of Treg cells in the spleen(6.83% ± 0.21% vs. 13.30% ± 0.35%;t = 15.65,P < 0.01)and LNs(8.26% ± 0.15% vs. 14.37% ± 0.44%;t =13.14,P<0.01)were lower in the normal mice than those in the infected mice.In addition,the proportions of Treg cells in the spleen(15.50%±0.76% vs.13.07%±0.15%;t=3.130,P<0.05)and LNs(17.00% ± 0.41% vs.13.83% ± 0.18%;t=6.947, P<0.01)were higher in the infected mice injected intraperitoneally with 2DG than those in the infected mice injected intraperi-toneally with PBS. Conclusion Glycolytic pathway inhibits Treg differentiation in the spleen and mesenteric LNs of S.japoni-cum-infected mice.
