Effects of donepezil on the expression of neurotrophic related factors in subventricular zone of cerebral infarction mice
10.3760/cma.j.issn.1674-6554.2018.11.001
- VernacularTitle:多奈哌齐对脑梗死模型小鼠室管膜下区神经发生相关营养因子表达的影响
- Author:
Yongxin ZHANG
1
;
Jianping WANG
;
Kefei CUI
;
Xiaojie FU
;
Di ZHANG
;
Zhengfang LU
;
Yufeng GAO
;
Jiang MAN
;
Xianliang LIU
;
Sijia LI
;
Linghui LIAO
;
Xiang LI
Author Information
1. 郑州大学第五附属医院神经内科
- Keywords:
Donepezil;
Cerebral infarction;
Subventricular zone;
Neurogenesis;
Neurotrophic factors
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2018;27(11):961-965
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of donepezil on subventricular zone ( SVZ) neuro-genesis related neurotrophic factors after cerebral infarction. Methods Mice were randomly assigned into three groups: vehicle-treated sham group (Sham+vehicle,n=18),vehicle-treated middle cerebral artery oc-clusion (MCAO) group (MCAO + vehicle,n=30) and donepezil-treated MCAO group (MCAO+donepezil, n=30). Middle cerebral artery occlusion( MCAO) was induced by thread-occlusion method. Nissl staining was used to measure the infarct volume and the modified neurological severity score(mNSS) was used to as-sess neurologic function and brain water content was detected to assess brain edema degree. Proliferative cells and neuroblasts were labeled with 5-bromodeoxyuridine ( BrdU) and doublecortin ( DCX). The SVZ BrdU+/DCX+cells were detected by immunofluorescence. The expression of glial cell line-derived neurotro-phic factor (GDNF),brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) were detec-ted by Western blot. Results The infarct volume of MCAO + donepezil group ((13. 33±4. 55)%) was sig-nificantly lower than that of MCAO + vehicle group ((31. 33±3. 93)%,t=7. 34,P<0. 05). The neurologic deficits were significantly ameliorated after donepezil treatment,and the brain water content of MCAO + done-pezil group ((71. 82±10. 18)%)was significantly less than that of MCAO + vehicle group ((85. 93± 7. 54)%,F=13. 480,P<0. 05). All differences were statistically significant (P<0. 05). The area of BrdU+/DCX+cells within SVZ of MCAO + vehicle group ((6. 16±1. 79)%) was significantly larger than that of sham + vehicle group ((2. 25±1. 09)%),and was fewer than that of MCAO+donepezil group ((16. 19± 2. 16)%,F=102. 756,P<0. 05). MCAO significantly promoted the expression of GDNF,BDNF and NGF within SVZ compared with sham operation,and donepezil increased these protein levels(F=15. 114,27. 121, 27. 398,P<0. 05). Conclusion Donepezil regulates neurogenesis via increasesing the expression of GDNF, BDNF and NGF within SVZ after cerebral infarction.
