Effects of resveratrol on cognitive dysfunction and the expression of parvalbumin in depression model mice
10.3760/cma.j.issn.1674-6554.2018.04.002
- VernacularTitle:白藜芦醇对抑郁模型小鼠认知功能及小清蛋白的影响
- Author:
Jia WANG
1
,
2
;
Lei WANG
;
Qi YUN
;
Huangrong SONG
;
Weining ZHANG
;
Yang JIANG
;
Jinjun QIAN
;
Xianlu CHANG
Author Information
1. 212013 镇江,江苏大学医学院检验系
2. 212000 镇江,江苏大学第四人民医院神经内科
- Keywords:
Resveratrol;
Depression;
Cognition;
SIRT1;
PGC-1α;
Parvalbumin;
Mice
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2018;27(4):294-299
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the antidepressant effects of resveratrol (RSV),and its possible mechanism specialized on improving cognitive function.Methods Thirty-two C57BL/6J mice were randomly divided into four groups:Control,Model,Model+RSV and Model+NA+RSV groups.The mice were subjected corticosterone (20 mg/(kg · d)) intraperitoneal injection for 21 consecutive days except the control mice.From the 22nd to 42nd day,the mice in different groups received further treatment with vehicle/ RSV (400 mg/(kg · d),op)/NA (100 mg/(kg · d),ip)+RSV (400 mg/(kg · d),op).The sugar preference test,novel object recognition test,novel location recognition test and water maze test were applied to evaluate the cognitive effects of RSV on mice.Subsequently,the silence information regulation factor 1 (SIRT1),peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α),parvalbumin (PV) transcription and translation level were evaluated by RT-PCR and Western blotting.Results The sugar preference test showed that the depression model mediated by CORT injection was successfully established(F（1,30） =6,P=0.038).In in the novel object learning test,resveratrol significantly increased the proration on the frequency ((-0.20±0.37) vs (0.16±0.29))and duration((0.10±0.45) vs (0.62±0.29)) and decreased the proration on the distance((0.09±0.36) vs (0.55±0.27)).In the water maze test,resveratrol reduced the time((41±9)s;(26± 8) s) and distance ((295± 70) cm;(224±43) cm) to find the platform.All the results were accompanied with the increased expression of protein SIRT1 (F(3,29) =15.60,P＜0.01),PGC-1α(F(3,29)=7.51,P=0.0006) and PV (F(3,29) =17.87,P=0.0004).While pretreatment with nicotinamide,resveratrol could not rescue the cognitive impairment and could not reverse the iecreased expression level of protein SIRT1,PGC-1 α and PV.Conelusion Resveratrol can reverse the cognitive dysfunction of depressant mice,which may be achieved by activating the SIRT1/PGC-1α signaling pathway and increasing the transcription and protein expression of PV.
