Expression of microRNA506 in brain tissue of mice with cerebral ischemia reperfusion injury and its effect on proliferation and apoptosis of SH-SY5Y cells
10.3760/cma.j.issn.1674-6554.2018.01.005
- VernacularTitle:microRNA506在脑缺血再灌注模型小鼠脑组织的表达及对SH-SY5Y细胞增殖和凋亡的影响
- Author:
Yifeng LIU
1
;
Changming WEN
;
Gongling WEN
;
Baozhao ZHANG
;
Wei LI
Author Information
1. 南阳市中心医院神经内科
- Keywords:
microRNA506;
Crebral ischemia reperfusion;
SH-SY5Y;
Cell proliferation;
Aoptosis;
Mice
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2018;27(1):22-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of microRNA506 in brain tissue of mice with cere-bral ischemia reperfusion injury and its effect on proliferation and apoptosis of SH-SY5Y cells. Method-s Middle cerebral artery occlusion(MCAO)was used to establish the model of cerebral ischemia reperfu-sion in mice,and RT-PCR(reverse transcription polymerase chain reaction)was used to detect the expres-sion level of microRNA506 in the brain tissue of mice. MicroRNA506 overexpression plasmids and interfer-ence plasmids were constructed and transfected into SH-SY5Y cells respectively.Cell apoptosis was detected by flow cytometry,and cell proliferation was detected by MTT assay. Results The level of microRNA506 in brain tissue of cerebral ischemia reperfusion model mice was significantly higher than that of sham operation group((51.16±1.64)vs(12.82±1.66),P=0.008). The siRNA targeting microRNA506 significantly re-duced the growth of SH-SY5Y cells(P=0.0072),and induced the apoptosis of SH-SY5Y cells(P=0.0073). Transfection of microRNA506 enhanced the proliferation rate(P=0.020)of SH-SY5Y cells,and reduced the apoptosis of SH-SY5Y cells(P=0.017).Conclusions The expression level of microRNA506 in brain tissue of mice with cerebral ischemia reperfusion is increased.Overexpression of microRNA506 promotes the cell proliferation and decreases the apoptosis rate of SH-SY5Y cell while the low expression of microRNA506 de-creases the growth rate and induce cell apoptosis of SH-SY5Y cell.
