Relationship Between Sterol Regulatory Element Binding Protein-1 and Nucleotide-binding Oligomerization Domain-like Receptor Protein-1 Inflammasome in Patients With Coronary Artery Disease
10.3969/j.issn.1000-3614.2018.03.005
- VernacularTitle:冠心病患者固醇调节元件结合蛋白-1与核苷酸结合寡聚化结构域样受体蛋白1炎性体相关性研究
- Author:
Yan-Jun PAN
1
;
Yan WANG
;
Jian-Qiang XU
;
Si-Yang YU
;
Gao-Feng ZENG
;
Guo-Jun ZHAO
Author Information
1. 南华大学附属第二医院 心血管内科
- Keywords:
Sterol regulatory element binding protein-1;
Nucleotide-binding oligomerization domain-like receptor protein-1 inflammasome;
Coronary artery disease;
Inflammation
- From:
Chinese Circulation Journal
2018;33(3):227-230
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the relationship between sterol regulatory element binding protein-1 (SREBP-1) and nucleotide-binding oligomerization domain-like receptor protein-1 (NLRP-1) inflammasome in patients with coronary artery disease (CAD). Methods: Our research included in 3 groups: Control group, n=20 normal subjects, SAP (stable angina pectoris) group, n=49 and ACS (acute coronary syndrome) group, n=55. Plasma levels of IL-1β, IL-18 and hs-CRP were examined by ELISA, mRNA and protein expressions of SREBP-1, NLRP-1 and Caspase-1 in peripheral blood mononuclear cells (PBMC) were detected by RT-PCR and Western blot analysis. Relationships between SREBP-1 and NLRP1, Caspase-1, IL-1β, IL-18 were studied. Results: Compared with Control group, ACS group and SAP group had increased plasma levels of IL-1β, IL-18 and hs-CRP, all P<0.05; elevated mRNA and protein expressions of SREBP-1, NLRP-1 and Caspase-1 in PBMC, in addition, those expressions in ACS group was even higher than SAP group, all P<0.05. SREBP-1 level was positively related to NLRP1, Caspase-1, IL-1β and IL-18, all P<0.05. Conclusion: The expressions of SREBP-1 and NLRP1 inflammasome were increased in CAD patients; SREBP-1 and NLRP1 had positive correlation.
