Micro RNA differential expression in primary retroperitoneal liposarcoma
10.3969/j.issn.1009-9905.2018.03.005
- VernacularTitle:Micro RNA在原发性腹膜后脂肪肉瘤组织中表达差异谱的研究
- Author:
Ya-Nan DONG
1
;
Fa-Bo QIU
;
Bing HAN
;
Bing-Qi YU
;
Chang-Li XU
;
Xiao-Feng SONG
;
Guang-Tao MA
Author Information
1. 青岛大学附属医院肝胆外科 山东青岛266003
- Keywords:
Micro RNA;
Primary retroperitoneal liposarcoma;
MiRNA chips
- From:
Chinese Journal of Current Advances in General Surgery
2018;21(3):186-190
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the miRNA (micro RNA)differential expression profile between primary retroperitoneal liposarcoma tissues and normal fat tissues,and to provide the evidence that miRNA were involved in the molecular pathways of primary retroperitoneal liposarcoma tissues' occurrence.Methods:Collecting retroperitoneal liposarcoma tissues and normal fat tissues from 4 patients after radical surgery of retroperitoneal lipsarcoma.Using microarray analysis.The tissues' miRNA hybridizated with human's LC Sciences microRNA Microarray-Single (miRBase 21.0) expression profile gene chips,and got the date.Analyzing the differential expressing of the siginal date by LOWESS.Results:Total 38 differential expressed miRNA were found (P＜0.05),including 23 over-expression and 15 low-expression miRNAs.10 of them(38 differential miRNAs) was significant deviation (P＜0.01),including 4 over-expression and 6 low-expression.Date analysis revealed that some miRNAs were associated some different tumors,Conclusion:The number of over-expression were more than the low-expression in primary retroperitoneal liposarcoma compared with the normol fat tissue,which indicate that the genes expression are less abundant in primary retroperitoneal li-posarcoma;some of the miRNAs might involved in the molecular pa-thways of primary retroperitoneal liposarcoma tissues' occurrence and recurrence,they might become the target point of the targetedtherapy of the primary retroperitoneal liposarcoma,some of the over-expressed miRNAs can become new biomarkers in the following diagnosis of the primary retroperitoneal liposarcoma.
