Effect of metformin on angiotensin Ⅱ-induced abdominal aortic aneurysm in ApoE-/-mice
10.3969/j.issn.1009-9905.2018.02.001
- VernacularTitle:二甲双胍对血管紧张素Ⅱ诱导的ApoE-/-小鼠腹主动脉瘤形成的影响
- Author:
Wei-Ce SUN
1
;
Xing JIN
Author Information
1. 山东大学附属山东省立医院 血管外科
- Keywords:
Abdominal aortic aneurysm;
Metformin;
Inflammation;
Nuclear factor Κb signaling
- From:
Chinese Journal of Current Advances in General Surgery
2018;21(2):85-89
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of metformin (MET) in inflammatory response in abdominal aortic aneurysm(AAA) formation induced by angiotensin Ⅱ.Methods:AAA models were established by Ang Ⅱ infusion in ApoE-/-mice.Thirty male ApoE-/-mice (8-10-week-old) were randomly and equally divided into four groups:Control group,AAA group and AAA+MET group.HE staining and immunohistochemical staining were used to analyze the inflammatory response.Real-time quantitative polymerase chain reaction (qPCR) was used to analyze the mRNA expression of intercellular cell adhesion molecule-1 (ICAM-1).Western blotting was used to analyze the activation of nuclear factor κB signaling (NF-κB).Results:The AAA incidence and mean maximum suprarenal aortic diameter of AAA group is larger than the AAA+MET group.Compared with the AAA grouP,the expression of ICAM-1 and activity of NF-κB is lower in AAA+MET group.Conclusion:Metformin can attenuate AAA formation partially by regulating macrophage infiltration through regulating the mRNA expression of ICAM-1 regulated by the activation of NF-κB signaling pathway.
