Expression of HSP90 in cholangiocarcinoma tissues and cells and the effect of 17-AAG on cholangiocarcinoma cell line
10.3969/j.issn.1009-9905.2017.12.003
- VernacularTitle:HSP90在胆管癌组织与细胞中的表达及17-AAG对胆管癌细胞株的影响
- Author:
Jing-Rui ZHANG
1
;
Feng GAO
;
Rong-Jiang LI
;
Zhen-Xiong QIU
;
Zhen-Hua HUANG
Author Information
1. 深圳市宝安区中心医院普通外科 广东 深圳 518102
- Keywords:
Cholangiocarcinoma;
HSP90;
17-AAG;
TE-1
- From:
Chinese Journal of Current Advances in General Surgery
2017;20(12):933-936
- CountryChina
- Language:Chinese
-
Abstract:
Objeetive:To investigate the expression of HSP90 in cholangiocarcinoma tissues and cells,and the effect of 17-AAG on cholangiocarcinoma cell line.Methods:Forty patients with cholangiocarcinoma admitted to our hospital from July 2015 to July 2016 were selected as study subjects.Expression of HSP90 in cholangiocarcinoma tissues,paracancerous tissues and normal tissues was detected respectively.Cholangiocarcinoma cell lines were treated with different concentrations of 17-AAG,and expression of HSP90 and its effect protein HIF-1 α was detected.R esults:The total positive rate in cholangiocarcinoma tissues (82.5％) was higher than paracancerous tissues (35.0％) and normal tissues (20.0％)(all P＜0.05).The strong positive expression rate in cholangiocarcinoma tissues(37.5％) was higher than paracancerous tissues(10.0％) and normal tissues (5.0％)(all P＜0.05).There was no significant correlation between expression of HSP90 in cholangiocarcinoma tissues and invasion depth,lymph node metastasis (P＞0.05),but there was a significant correlation with TNM stage and tumor diameter (P＜0.05).With the increase of treatment concentration of 17-AAG,HSP90 α / β-actin and HIF-1 α / β-actin decreased,and there were significant differences in expression of HSP90α and its effect protein HIF-1 α in cholangiocarcinoma cell lines under different treatment concentrations (P＜0.05).Conclusion:HSP90 is highly expressed in cholangiocarcinoma tissues and cells and its expression is related to clinical stage and tumor diameter.17-AAG can down regulates the expression of HSP90 α and HIF-1 α in TE-1 cells,which plays a certain guiding role in clinical treatment.
